MersV1, Main, Exploration, bibRecord, 002D41

DNA and RNA as new binding targets of green tea catechins.

Identifieur interne : 002D41 ( Main/Exploration ); précédent : 002D40; suivant : 002D42

DNA and RNA as new binding targets of green tea catechins.

Auteurs : Takashi Kuzuhara [Japon] ; Yoshihisa Sei ; Kentaro Yamaguchi ; Masami Suganuma ; Hirota Fujiki

Source :

The Journal of biological chemistry [ 0021-9258 ] ; 2006.

RBID : pubmed:16641087

Descripteurs français

KwdFr :
- ADN (), ADN simple brin (), ARN (), Altération de l'ADN, Apoptose, Catéchine (), Catéchine (analogues et dérivés), Modèles chimiques, Modèles moléculaires, Nucléotides (), Piégeurs de radicaux libres (), Résonance plasmonique de surface (), Spectrométrie de masse ESI (), Température, Thé.
MESH :
- analogues et dérivés : Catéchine.
- ADN, ADN simple brin, ARN, Altération de l'ADN, Apoptose, Catéchine, Modèles chimiques, Modèles moléculaires, Nucléotides, Piégeurs de radicaux libres, Résonance plasmonique de surface, Spectrométrie de masse ESI, Température, Thé.

English descriptors

KwdEn :
- Apoptosis, Catechin (analogs & derivatives), Catechin (chemistry), DNA (chemistry), DNA Damage, DNA, Single-Stranded (chemistry), Free Radical Scavengers (chemistry), Models, Chemical, Models, Molecular, Nucleotides (chemistry), RNA (chemistry), Spectrometry, Mass, Electrospray Ionization (methods), Surface Plasmon Resonance (methods), Tea, Temperature.
MESH :
- chemical , analogs & derivatives : Catechin.
- chemical , chemistry : Catechin, DNA, DNA, Single-Stranded, Free Radical Scavengers, Nucleotides, RNA.
- methods : Spectrometry, Mass, Electrospray Ionization, Surface Plasmon Resonance.
- Apoptosis, DNA Damage, Models, Chemical, Models, Molecular, Tea, Temperature.

Abstract

The significance of catechins, the main constituent of green tea, is being increasingly recognized with regard to cancer prevention. Catechins have been studied for interactions with various proteins, but the mechanisms of the various catechins are not yet elucidated. Based on our previous observation that nucleic acids extracted from catechin-treated cells are colored, we studied whether catechins directly interact with nucleic acids using surface plasmon resonance assay (Biacore) and cold spray ionization-mass spectrometry. These two methods clearly showed that (-)-epigallocatechin gallate (EGCG) binds to both DNA and RNA molecules: the Biacore assay indicated that four catechins bound to DNA oligomers, and cold spray ionization-mass spectrometry analysis showed one to three EGCG molecules bound to single strand 18 mers of DNA and RNA. Moreover, one or two molecules of EGCG bound to double-stranded (AG-CT) oligomers of various nucleotide lengths. These results suggest that multiple binding sites of EGCG are present in DNA and RNA oligomers. Double-stranded DNA (dsDNA) oligomers were detected only as EGCG-bound forms at high temperature, whereas at low temperature both the free and bound forms were detected, suggesting that EGCG protects dsDNA oligomers from dsDNA melting to single-stranded DNA. Because both galloyl and catechol groups of EGCG are essential for DNA binding, both groups seem to hold strands of DNA via their branching structure. These findings reveal for the first time the link between catechins and polynucleotides and will intensify our understanding of the effects of catechins on DNA in terms of cancer prevention.

DOI: 10.1074/jbc.M601196200
PubMed: 16641087

Affiliations:

Japon

Le document en format XML

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<term>DNA (chemistry)</term>
<term>DNA Damage</term>
<term>DNA, Single-Stranded (chemistry)</term>
<term>Free Radical Scavengers (chemistry)</term>
<term>Models, Chemical</term>
<term>Models, Molecular</term>
<term>Nucleotides (chemistry)</term>
<term>RNA (chemistry)</term>
<term>Spectrometry, Mass, Electrospray Ionization (methods)</term>
<term>Surface Plasmon Resonance (methods)</term>
<term>Tea</term>
<term>Temperature</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN ()</term>
<term>ADN simple brin ()</term>
<term>ARN ()</term>
<term>Altération de l'ADN</term>
<term>Apoptose</term>
<term>Catéchine ()</term>
<term>Catéchine (analogues et dérivés)</term>
<term>Modèles chimiques</term>
<term>Modèles moléculaires</term>
<term>Nucléotides ()</term>
<term>Piégeurs de radicaux libres ()</term>
<term>Résonance plasmonique de surface ()</term>
<term>Spectrométrie de masse ESI ()</term>
<term>Température</term>
<term>Thé</term>
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<term>Free Radical Scavengers</term>
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<term>Surface Plasmon Resonance</term>
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<term>DNA Damage</term>
<term>Models, Chemical</term>
<term>Models, Molecular</term>
<term>Tea</term>
<term>Temperature</term>
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<term>ADN simple brin</term>
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<term>Modèles moléculaires</term>
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<term>Piégeurs de radicaux libres</term>
<term>Résonance plasmonique de surface</term>
<term>Spectrométrie de masse ESI</term>
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<front><div type="abstract" xml:lang="en">The significance of catechins, the main constituent of green tea, is being increasingly recognized with regard to cancer prevention. Catechins have been studied for interactions with various proteins, but the mechanisms of the various catechins are not yet elucidated. Based on our previous observation that nucleic acids extracted from catechin-treated cells are colored, we studied whether catechins directly interact with nucleic acids using surface plasmon resonance assay (Biacore) and cold spray ionization-mass spectrometry. These two methods clearly showed that (-)-epigallocatechin gallate (EGCG) binds to both DNA and RNA molecules: the Biacore assay indicated that four catechins bound to DNA oligomers, and cold spray ionization-mass spectrometry analysis showed one to three EGCG molecules bound to single strand 18 mers of DNA and RNA. Moreover, one or two molecules of EGCG bound to double-stranded (AG-CT) oligomers of various nucleotide lengths. These results suggest that multiple binding sites of EGCG are present in DNA and RNA oligomers. Double-stranded DNA (dsDNA) oligomers were detected only as EGCG-bound forms at high temperature, whereas at low temperature both the free and bound forms were detected, suggesting that EGCG protects dsDNA oligomers from dsDNA melting to single-stranded DNA. Because both galloyl and catechol groups of EGCG are essential for DNA binding, both groups seem to hold strands of DNA via their branching structure. These findings reveal for the first time the link between catechins and polynucleotides and will intensify our understanding of the effects of catechins on DNA in terms of cancer prevention.</div>
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Serveur d'exploration MERS

DNA and RNA as new binding targets of green tea catechins.

DNA and RNA as new binding targets of green tea catechins.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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Pour générer des pages wiki

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